Journal: ACS Bio & Med Chem Au

Article Title: High Drug Encapsulation Capacity in Cyclodextrin-Based Nanoparticles: Characterization and In Vivo Antitumor Efficacy in Tumor-Bearing Mice

doi: 10.1021/acsbiomedchemau.5c00220

Figure Lengend Snippet: (A) Cryo-TEM images of MGS encapsulated in CDNP_PEG_4. The scale bar is 200 nm. (B) The scale bar is 50 nm. SAXS measurement of CDNP with Encapsulated MGS. (C) Comparison of SAXS profiles with and without MGS encapsulation in CDNP_PEGs and CDNP_ECH with MGS encapsulation. (D) SAXS results with different feeding concentration of MGS. Change in drug encapsulation ratio in (E) and [MGS]/[CD] in (F) as a function of MGS concentration. (G) Relationship between CD density ratio and [MGS]/[CD] in particles. (H) The mechanism of structural changes anticipated with drug encapsulation. Data are presented from a single experiment ( n = 1).

Article Snippet: Cryogenic transmission electron microscopy (Cryo-TEM) was performed using a JEM-2100Plus electron microscope (JEOL, Tokyo, Japan) operated at an accelerating voltage of 200 kV.

Techniques: Comparison, Encapsulation, Concentration Assay

Journal: Pharmaceutics

Article Title: A Polysorbate-Based Lipid Nanoparticle Vaccine Formulation Induces In Vivo Immune Response Against SARS-CoV-2

doi: 10.3390/pharmaceutics17040441

Figure Lengend Snippet: Figure 6. mRNA purity and in vitro transfection efficiency analyses of Fluc-PS80 LNP formulations containing various lipid combinations prepared in TSS buffer. (a) No substantial difference was observed between formulations in terms of mRNA purity, and (b) in vitro transfection efficiency was reduced with increased DSPC and decreased Dlin-MC3-DMA content. While F4–F6 formulations containing 1.5 mol% PS-80 showed higher transfection efficiency as compared to F1–F3 formulations, it significantly reduced following freeze/thaw. The formulations following freeze/thaw were significantly different in the mean luminescence intensity. The represented data are mean ± standard deviation of three independent measurements. Statistical comparison between samples was analyzed using Two-Way ANOVA with Bonferroni post hoc. ** p value <0.05, *** p value < 0.001, **** p value < 0.0001.

Article Snippet: Abbreviations The following abbreviations are used in this manuscript: Cryo-TEM Cryogenic transmission electron microscopy DLin-MC3-DMA [(6Z,9Z,28Z,31Z)-heptatriaconta-6,9,28,31-tetraen-19-yl]4-(dimethylamino)butanoate DMEM Dulbecco’s Modified Eagle’s Medium DMG-PEG2K LNP Fluc mRNA-LNP containing DMG-PEG2K as the PEG-lipid DMG-PEG2K 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 DSPC Distearoylphosphatidylcholine FBS Fetal bovine serum FLuc mRNA Firefly luciferase mRNA Fluc-PS80 LNP Firefly luciferase reporter mRNA-LNP HEK-293 cells Human embryonic kidney 293 cells LNPs Lipid nanoparticles MCE Microchip capillary electrophoresis PS-80 Polysorbate-80 PSS Phosphate-saline-sucrose buffer pVNT50 50% pseudovirus neutralization titer SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 SC2-PS80 LNP Polysorbate 80-based SARS-CoV-2 mRNA-LNP TSS Tris-saline-sucrose buffer References 1.

Techniques: In Vitro, Transfection, Standard Deviation, Comparison

Journal: Pharmaceutics

Article Title: A Polysorbate-Based Lipid Nanoparticle Vaccine Formulation Induces In Vivo Immune Response Against SARS-CoV-2

doi: 10.3390/pharmaceutics17040441

Figure Lengend Snippet: Figure 5. Representative cryo-TEM images of four Fluc-PS80 LNP formulations prepared in TSS buffer. (a) Fresh formulations, and (b) following one freeze/thaw cycle at −80 ◦C. For fresh formulations, F4–F6 formulations containing 1.5 mol% PS-80 showed a higher number of particles as compared to the F1 formulation containing 3 mol% PS-80. Increasing DSPC and simultaneously decreasing Dlin-MC3-DMA content resulted in changed particle morphology from circular to elongated LNPs with polyhedral shapes. For formulations subjected to freeze/thaw, F5 and F6 formulations showed a significant increase in the size of the particles as compared to the F1 formulation. The scale bar shows 200 nm for all presented images.

Article Snippet: Abbreviations The following abbreviations are used in this manuscript: Cryo-TEM Cryogenic transmission electron microscopy DLin-MC3-DMA [(6Z,9Z,28Z,31Z)-heptatriaconta-6,9,28,31-tetraen-19-yl]4-(dimethylamino)butanoate DMEM Dulbecco’s Modified Eagle’s Medium DMG-PEG2K LNP Fluc mRNA-LNP containing DMG-PEG2K as the PEG-lipid DMG-PEG2K 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 DSPC Distearoylphosphatidylcholine FBS Fetal bovine serum FLuc mRNA Firefly luciferase mRNA Fluc-PS80 LNP Firefly luciferase reporter mRNA-LNP HEK-293 cells Human embryonic kidney 293 cells LNPs Lipid nanoparticles MCE Microchip capillary electrophoresis PS-80 Polysorbate-80 PSS Phosphate-saline-sucrose buffer pVNT50 50% pseudovirus neutralization titer SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 SC2-PS80 LNP Polysorbate 80-based SARS-CoV-2 mRNA-LNP TSS Tris-saline-sucrose buffer References 1.

Techniques: Formulation